A highly sensitive, nested polymerase chain reaction based method using simple DNA extraction to detect malaria sporozoites in mosquitos
Vythilingam, I, Nitiavathy, K, Yi, P, Bakotee, B, Hugo, B, Singh, B, Wirtz, RA, Palmer, K
Southeast Asian J Trop Med Public Health 30(4), 631-5. (1999-12)
Dried Anopheles farauti mosquitos caught in Solomon Islands in 1990 were examined for malaria sporozoites by ELISA and nested polymerase chain reaction (PCR). Only heads and thoraces were used. Plasmodium genus-specific nested PCR amplifications were carried out on all samples. Of the 402 pools of mosquitos that were processed, 30 were positive for...